Improved compensation in flow cytometry by multivariable optimization

Improved measurement of calcium mobilization by flow cytometry.

Spectral compensation for flow cytometry: visualization artifacts, limitations, and caveats.

BACKGROUND In multicolor flow cytometric analysis, compensation for spectral overlap is nearly always necessary. For the most part, such compensation has been relatively simple, producing the desired rectilinear distributions. However, in the realm of multicolor analysis, visualization of compensated often results in unexpected distributions, principally the appearance of a large number of even...

Data-Driven Compensation for Flow Cytometry of Solid Tissues

Propidium Iodide is a fluorochrome that is used to measure the DNA content of individual cells, taken from solid tissues, with a flow cytometer. Compensation for spectral cross-over of this fluorochrome still leads to compensation results that are depending on operator experience. We present a data-driven compensation (DDC) algorithm that is designed to automatically compensate combined DNA phe...

Evaluation of DNA-content of Peritoneal Lavage Cells by Flow Cytometry in Patients with Gasteric Cancer

Background & Aims: Gastric cancer is the second common cancer in Iran and it has unfortunately no diagnostic signs in early stages. Considering the role of genetic and environmental factors in the development of gastric cancer, this study aimed at evaluating DNA-content of stomach cells in patients with gastric cancer in order to find a reliable index for prediction of disease prognosis and pro...

Functional Assays by Flow Cytometry

Cell function assays have been redefined over the past several years, largely because of technologies such as flow cytometry. The major difference between other techniques and those proposed using flow cytometry is the ability to analyze viable, single cells in a rapid and quantitative fashion. One of the advantages of flow cytometry methods lies in the ability to obtain population information....